PsSAK1, a stress-activated MAP kinase of Phytophthora sojae, is required for zoospore viability and infection of soybean.
Identifieur interne : 001850 ( Main/Exploration ); précédent : 001849; suivant : 001851PsSAK1, a stress-activated MAP kinase of Phytophthora sojae, is required for zoospore viability and infection of soybean.
Auteurs : Aining Li [République populaire de Chine] ; Yonglin Wang ; Kai Tao ; Suomeng Dong ; Qian Huang ; Tingting Dai ; Xiaobo Zheng ; Yuanchao WangSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2010.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Extinction de l'expression des gènes (MeSH), Maladies des plantes (microbiologie), Mitogen-Activated Protein Kinases (génétique), Mitogen-Activated Protein Kinases (métabolisme), Phytophthora (croissance et développement), Phytophthora (enzymologie), Phytophthora (pathogénicité), Phénotype (MeSH), Protéines d'algue (génétique), Protéines d'algue (métabolisme), Soja (microbiologie), Transcription génétique (MeSH), Virulence (MeSH).
- MESH :
- croissance et développement : Phytophthora.
- enzymologie : Phytophthora.
- génétique : Mitogen-Activated Protein Kinases, Protéines d'algue.
- microbiologie : Maladies des plantes, Soja.
- métabolisme : Mitogen-Activated Protein Kinases, Protéines d'algue.
- pathogénicité : Phytophthora.
- Analyse de profil d'expression de gènes, Extinction de l'expression des gènes, Phénotype, Transcription génétique, Virulence.
English descriptors
- KwdEn :
- Algal Proteins (genetics), Algal Proteins (metabolism), Gene Expression Profiling (MeSH), Gene Silencing (MeSH), Mitogen-Activated Protein Kinases (genetics), Mitogen-Activated Protein Kinases (metabolism), Phenotype (MeSH), Phytophthora (enzymology), Phytophthora (growth & development), Phytophthora (pathogenicity), Plant Diseases (microbiology), Soybeans (microbiology), Transcription, Genetic (MeSH), Virulence (MeSH).
- MESH :
- chemical , genetics : Algal Proteins, Mitogen-Activated Protein Kinases.
- chemical , metabolism : Algal Proteins, Mitogen-Activated Protein Kinases.
- enzymology : Phytophthora.
- growth & development : Phytophthora.
- microbiology : Plant Diseases, Soybeans.
- pathogenicity : Phytophthora.
- Gene Expression Profiling, Gene Silencing, Phenotype, Transcription, Genetic, Virulence.
Abstract
Mitogen-activated protein kinase (MAPK) pathways are universal and evolutionarily conserved signal transduction modules in all eukaryotic cells. In this study, PsSAK1, which encodes a stress-activated MAPK of Phytophthora sojae, was identified. PsSAK1 is highly conserved in oomycetes, and it represents a novel group of MAPK due to its pleckstrin homology domain. Reverse-transcription polymerase chain reaction analysis showed that PsSAK1 expression was upregulated in zoospores and cysts and during early infection. In addition, its expression was induced by osmotic and oxidative stress mediated by NaCl and H(2)O(2), respectively. To elucidate the function, the expression of PsSAK1 was silenced using stable transformation of P. sojae. The silencing of PsSAK1 did not impair hyphal growth, sporulation, or oospore production but severely hindered zoospore development, in that the silenced strains showed quicker encystment and a lower germination ratio than the wild type. PsSAK1-silenced mutants produced much longer germ tubes and could not colonize either wounded or unwounded soybean leaves. Our results indicate that PsSAK1 is an important regulator of zoospore development and pathogenicity in P. sojae.
DOI: 10.1094/MPMI-23-8-1022
PubMed: 20615113
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">PsSAK1, a stress-activated MAP kinase of Phytophthora sojae, is required for zoospore viability and infection of soybean.</title><author><name sortKey="Li, Aining" sort="Li, Aining" uniqKey="Li A" first="Aining" last="Li">Aining Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yonglin" sort="Wang, Yonglin" uniqKey="Wang Y" first="Yonglin" last="Wang">Yonglin Wang</name></author><author><name sortKey="Tao, Kai" sort="Tao, Kai" uniqKey="Tao K" first="Kai" last="Tao">Kai Tao</name></author><author><name sortKey="Dong, Suomeng" sort="Dong, Suomeng" uniqKey="Dong S" first="Suomeng" last="Dong">Suomeng Dong</name></author><author><name sortKey="Huang, Qian" sort="Huang, Qian" uniqKey="Huang Q" first="Qian" last="Huang">Qian Huang</name></author><author><name sortKey="Dai, Tingting" sort="Dai, Tingting" uniqKey="Dai T" first="Tingting" last="Dai">Tingting Dai</name></author><author><name sortKey="Zheng, Xiaobo" sort="Zheng, Xiaobo" uniqKey="Zheng X" first="Xiaobo" last="Zheng">Xiaobo Zheng</name></author><author><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20615113</idno><idno type="pmid">20615113</idno><idno type="doi">10.1094/MPMI-23-8-1022</idno><idno type="wicri:Area/Main/Corpus">001879</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001879</idno><idno type="wicri:Area/Main/Curation">001879</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001879</idno><idno type="wicri:Area/Main/Exploration">001879</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">PsSAK1, a stress-activated MAP kinase of Phytophthora sojae, is required for zoospore viability and infection of soybean.</title><author><name sortKey="Li, Aining" sort="Li, Aining" uniqKey="Li A" first="Aining" last="Li">Aining Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yonglin" sort="Wang, Yonglin" uniqKey="Wang Y" first="Yonglin" last="Wang">Yonglin Wang</name></author><author><name sortKey="Tao, Kai" sort="Tao, Kai" uniqKey="Tao K" first="Kai" last="Tao">Kai Tao</name></author><author><name sortKey="Dong, Suomeng" sort="Dong, Suomeng" uniqKey="Dong S" first="Suomeng" last="Dong">Suomeng Dong</name></author><author><name sortKey="Huang, Qian" sort="Huang, Qian" uniqKey="Huang Q" first="Qian" last="Huang">Qian Huang</name></author><author><name sortKey="Dai, Tingting" sort="Dai, Tingting" uniqKey="Dai T" first="Tingting" last="Dai">Tingting Dai</name></author><author><name sortKey="Zheng, Xiaobo" sort="Zheng, Xiaobo" uniqKey="Zheng X" first="Xiaobo" last="Zheng">Xiaobo Zheng</name></author><author><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algal Proteins (genetics)</term><term>Algal Proteins (metabolism)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Silencing (MeSH)</term><term>Mitogen-Activated Protein Kinases (genetics)</term><term>Mitogen-Activated Protein Kinases (metabolism)</term><term>Phenotype (MeSH)</term><term>Phytophthora (enzymology)</term><term>Phytophthora (growth & development)</term><term>Phytophthora (pathogenicity)</term><term>Plant Diseases (microbiology)</term><term>Soybeans (microbiology)</term><term>Transcription, Genetic (MeSH)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Extinction de l'expression des gènes (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Mitogen-Activated Protein Kinases (génétique)</term><term>Mitogen-Activated Protein Kinases (métabolisme)</term><term>Phytophthora (croissance et développement)</term><term>Phytophthora (enzymologie)</term><term>Phytophthora (pathogénicité)</term><term>Phénotype (MeSH)</term><term>Protéines d'algue (génétique)</term><term>Protéines d'algue (métabolisme)</term><term>Soja (microbiologie)</term><term>Transcription génétique (MeSH)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Algal Proteins</term><term>Mitogen-Activated Protein Kinases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Algal Proteins</term><term>Mitogen-Activated Protein Kinases</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Mitogen-Activated Protein Kinases</term><term>Protéines d'algue</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Soja</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Mitogen-Activated Protein Kinases</term><term>Protéines d'algue</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Gene Silencing</term><term>Phenotype</term><term>Transcription, Genetic</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Extinction de l'expression des gènes</term><term>Phénotype</term><term>Transcription génétique</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mitogen-activated protein kinase (MAPK) pathways are universal and evolutionarily conserved signal transduction modules in all eukaryotic cells. In this study, PsSAK1, which encodes a stress-activated MAPK of Phytophthora sojae, was identified. PsSAK1 is highly conserved in oomycetes, and it represents a novel group of MAPK due to its pleckstrin homology domain. Reverse-transcription polymerase chain reaction analysis showed that PsSAK1 expression was upregulated in zoospores and cysts and during early infection. In addition, its expression was induced by osmotic and oxidative stress mediated by NaCl and H(2)O(2), respectively. To elucidate the function, the expression of PsSAK1 was silenced using stable transformation of P. sojae. The silencing of PsSAK1 did not impair hyphal growth, sporulation, or oospore production but severely hindered zoospore development, in that the silenced strains showed quicker encystment and a lower germination ratio than the wild type. PsSAK1-silenced mutants produced much longer germ tubes and could not colonize either wounded or unwounded soybean leaves. Our results indicate that PsSAK1 is an important regulator of zoospore development and pathogenicity in P. sojae.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20615113</PMID><DateCompleted><Year>2010</Year><Month>09</Month><Day>13</Day></DateCompleted><DateRevised><Year>2010</Year><Month>07</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>23</Volume><Issue>8</Issue><PubDate><Year>2010</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>PsSAK1, a stress-activated MAP kinase of Phytophthora sojae, is required for zoospore viability and infection of soybean.</ArticleTitle><Pagination><MedlinePgn>1022-31</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-23-8-1022</ELocationID><Abstract><AbstractText>Mitogen-activated protein kinase (MAPK) pathways are universal and evolutionarily conserved signal transduction modules in all eukaryotic cells. In this study, PsSAK1, which encodes a stress-activated MAPK of Phytophthora sojae, was identified. PsSAK1 is highly conserved in oomycetes, and it represents a novel group of MAPK due to its pleckstrin homology domain. Reverse-transcription polymerase chain reaction analysis showed that PsSAK1 expression was upregulated in zoospores and cysts and during early infection. In addition, its expression was induced by osmotic and oxidative stress mediated by NaCl and H(2)O(2), respectively. To elucidate the function, the expression of PsSAK1 was silenced using stable transformation of P. sojae. The silencing of PsSAK1 did not impair hyphal growth, sporulation, or oospore production but severely hindered zoospore development, in that the silenced strains showed quicker encystment and a lower germination ratio than the wild type. PsSAK1-silenced mutants produced much longer germ tubes and could not colonize either wounded or unwounded soybean leaves. Our results indicate that PsSAK1 is an important regulator of zoospore development and pathogenicity in P. sojae.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Aining</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yonglin</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Tao</LastName><ForeName>Kai</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Suomeng</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Qian</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Dai</LastName><ForeName>Tingting</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Xiaobo</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yuanchao</ForeName><Initials>Y</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020418">Algal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.24</RegistryNumber><NameOfSubstance UI="D020928">Mitogen-Activated Protein Kinases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020418" MajorTopicYN="N">Algal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="N">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020928" MajorTopicYN="N">Mitogen-Activated Protein Kinases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>9</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20615113</ArticleId><ArticleId IdType="doi">10.1094/MPMI-23-8-1022</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Dai, Tingting" sort="Dai, Tingting" uniqKey="Dai T" first="Tingting" last="Dai">Tingting Dai</name><name sortKey="Dong, Suomeng" sort="Dong, Suomeng" uniqKey="Dong S" first="Suomeng" last="Dong">Suomeng Dong</name><name sortKey="Huang, Qian" sort="Huang, Qian" uniqKey="Huang Q" first="Qian" last="Huang">Qian Huang</name><name sortKey="Tao, Kai" sort="Tao, Kai" uniqKey="Tao K" first="Kai" last="Tao">Kai Tao</name><name sortKey="Wang, Yonglin" sort="Wang, Yonglin" uniqKey="Wang Y" first="Yonglin" last="Wang">Yonglin Wang</name><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name><name sortKey="Zheng, Xiaobo" sort="Zheng, Xiaobo" uniqKey="Zheng X" first="Xiaobo" last="Zheng">Xiaobo Zheng</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Li, Aining" sort="Li, Aining" uniqKey="Li A" first="Aining" last="Li">Aining Li</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001850 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001850 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20615113
|texte= PsSAK1, a stress-activated MAP kinase of Phytophthora sojae, is required for zoospore viability and infection of soybean.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20615113" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |